Membrane lauryl sulphate method with in situ urease test for Escherichia coli 膜法脱月桂硫酸盐法附以在原地测试尿素?内含的大肠杆菌
Hp infection was diagnosed by rapid urease test with gastric mucosa, 13C expiration test and serum Hp-IgG test by ELISA. 采用胃黏膜快速尿素酶实验、Hp-13C呼气试验和血清Hp-IgGELISA检测幽门螺旋杆菌。
Hp infection was identified by rapid urease test and histology. Hp感染采用快速尿素酶试验和组织学检查来判定。
It is supposed that the rapid urease test in combination with direct smear examination or the W-S silver method could reveal the positive rate more accurately. 作者认为,应用尿素酶试验同时进行直接涂片法或W-S银染法检查,可进一步提高阳性率。
PCR Method Compared with Rapid Urease Test to Detect Helicobacter Pylori PCR法及快速尿素酶试验检测幽门螺杆菌感染的比较
One and six months after the therapy C 14 urease breath test were used to evaluate the bacterial eradication rate of HP infections, and combined with endoscopy and urease test if necessary. 停药后1月和6个月进行随访,随访时对患者行C14呼吸试验确定HP根除率,必要时进行胃镜和尿素酶试验。
After four-week treatment they were examined through gastroscope, histology biopsy diagnosis and rapid urease test. 疗程结束后4周复查胃镜,胃黏膜活检及快速尿素酶试验。
Methods: Urease test and PCR were used. 方法:采用脲酶实验及PCR方法检测幽门螺杆菌。
All mice were killed after 4 weeks since last intragastric administration. Hp was detected by rapid urease test, Giemsa dying, and bacterial culture, and histopathologic changes in the gastric mucosa of mice were determined by H-E staining. 最后一次灌胃4周后处死小鼠,HE染色判断小鼠胃黏膜组织学损伤,并用快速尿素酶试验、Giemsa染色和细菌培养进行Hp的检测。
Rapid urease test and serum H.pylori antibodies with ELISA or Warthin-Starry silver stains were used for H.pylori diagnosis. pylori的诊断采用快速尿素酶试验和ELISA法血清H.pylori抗体或病理Warthin-Starry银染色,两项阳性诊断为H。
RESULTS: ① The mouse-model of Hp infecting gastric membrane: the gastric membrane of mice at 4 and 8 weeks after infection were positive in smear-microscopy, urease test and Warthin-Starry stain. The infection rate of Hp was 100%. 结果:①昆明系小鼠胃黏膜幽门螺杆菌感染模型:实验小鼠在感染4和8周后,胃黏膜涂片镜检、尿素酶试验及组织病理学Warthin-Starry染色检查均阳性,幽门螺杆菌感染率100%。
Hp was detected by rapid urease test and Warthin starry staining. 以快速尿素酶试验和Warthinstarry法检测组织中的Hp;
On the other hand, all patients who underwent endoscopy were studied with rapid urease test and histology at the same time. Most of the patients were also tested with serology and 13C-urea breath test ( 13C-UBT). 并利用胃镜取活组织行快速尿素酶试验和病理切片染色,大部分病例还结合血清学和13C尿素呼气试验(13C-UBT)。
Methods The diagnosis of chronic gastritis accords with updated Sydney classification system, rapid urease test, histopathological staining method and 13 C breath test were used in detection of HP. 方法慢性胃炎诊断标准参照最新悉尼分类系统,幽门螺杆菌检测采用快速尿素酶试验、病理组织学染色及13C呼气试验。
Methods Hp was detected by using rapid urease test and Warthin-Starry silver stain. Inducible nitric oxide synthase ( iNOS) was detected by using immunohistochemistry. 方法采用快速尿素酶试验及Warthin-Starry银染色法检测入选病例的Hp,用免疫组织化学染色法检测iNOS。
H.pylori was determined with the rapid urease test, Warthin-Starry method or 14C urease breath test; 采用Warthin-Starry染色、快速尿素酶试验或C14尿素呼气试验检测HP的感染情况;
Objective: To evaluate the effect of rapid urease test ( RUT) and~ ( 14) C-urea breath test ( 14C-UBT) in detecting Helicobacter pylori. 目的:评估14C-尿素呼气试验(14C-UBT)和快速尿素酶试验(RUT)检测幽门螺杆菌(Hp)的临床价值。
METHODS Hp in gastric fluid and dental plaque was detected with fluorescent antibody method, bacterial culture, urease test and Hp diagnosis card at the same time in 62 cases with gastric and duodenal disease. The gather of gastric fluid applied the capsule method. 方法应用荧光抗体方法、细菌培养、尿素酶试验、Hp诊断卡4种方法对62例胃、十二指肠疾病患者胃液、口腔牙菌斑进行Hp检测,胃液采集采用胶囊采样法。
Comparison of biopsy urease test, culture and histological examination in the diagnosis of Campylobacter pylori infection 快速尿素酶试验与细菌培养及组织学检查对诊断幽门弯曲菌感染的比较研究
Columbia blood agar was used as selective medium to isolate H. pylori. Gram-staining microscopy, urease test and Oxidase test were applied to identify the isolated bacteria. 采用选择性哥伦比亚血琼脂为分离培养基,通过革兰染色镜检、尿素酶试验和氧化酶试验对分离的幽门螺杆菌进行鉴定。
Biochemical identification showed that the bacteria were positive for repid urease test, oxidase test, peroxidase experiment and nitrate reductase test. 生化鉴定示细菌尿素酶实验阳性,氧化酶、过氧化物酶实验阳性,硝酸还原酶阴性。